Workshop on Technologies on molecular Analysis of cell and cellular Dynamics (TMCCD)
(19th November to 21st November 2015)
ABOUT THE WORKSHOP :
The workshop on “Technologies on molecular Analysis of cell and cellular Dynamics (TMACCD)” aims to extend its technical knowledge to the young researchers and students in the field of Life Science by giving them hands on training to participants. NIT Rourkela is one of the premier national level institutions for technical education in the country and is funded by the Government of India. The department of Life Science is actively engaged in teaching and research. Our department is newly established and came into existence in 2008 and the academic curriculum was fully functional in 2009. However, it is not new in terms of experience and cultivating science. The department is well equipped with all the advanced and necessary Instruments required to conduct high level research. Our faculties have the expertise in different fields like Biochemistry, Bioinformatics, Biophysics, Cell Biology, Immunology, Food science, Microbiology and molecular biology, Plant science, Developmental Biology. With the previous success of organizing 2nd international conference in our department in year 2015 (InCoFIBS-II), we are happy to announce this three day workshop, TMCCD.
- Invited lecture I
- Invited lecture II
- Invited lecture III
Module I: Gene Identification
Experiment 1.1 Isolation of total RNA from plant by TRIzol reagent. DNase I treatment, Purification, cDNA preparation.
Experiment 1.2 Primer Designing, RT-PCR, Ligation in pGEMT-Easy Vector, Transformation
Experiment 1.3 Blue/White colony Checking, Colony Lysis PCR, Gel Run, BLAST-N, BLAST-P, BLAST-X
Module II: Gene Expression Analysis
Experiment 2.1 Transfection of GFP tagged gene in Human cell
Experiment 2.2 Expression analysis by fluorescence microscope
Experiment 2.3 Expression analysis by western blot
Module III: Functional Analysis of Gene
Experiment 3.1 Propidium Iodide staining
Experiment 3.2 Cell cycle analysis
Experiment 3.3 Annexin V
Experiment 3.4 Acrydine orange staining
Experiment 3.5 DAPI staining
Experiment 3.6 Morphological study under SEM
Experiment 3.7 Behavioural study under Stereo-zoom microscope
WHO CAN APPLY:
Research scholars, graduates and post graduate students working in the areas of Life Science can apply. Mostly early career PhD/research students will be preferred as the seats are limited.
VENUE :
The workshop will be held on 19th-21st, November 2015 at Department of Life Science, National Institute of Technology, Rourkela, Odisha.